Fig. 2

Nile Red and LysoTracker Red staining of TSD NSCs. a Images of increased intensity of Nile Red staining in TSD NSC compared to WT NSCs after 24 h addition of 10% FBS. The yellow/gold fluorescence of Nile Red excites and emits at 450-500 nm and 528 nm, respectively. b LysoTracker Red staining in TSD NSCs. c Intensity of Nile Red staining in TSD NSCs after FBS treatment. d Intensity of LysoTracker Red staining in Tay-Sachs disease NSCs after FBS treatment. Data are displayed as mean ± SD. **** p < 0.0001 compared to WT control. IxA, integrated cell intensity