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Table 4 Summary of recombinant lysosomal enzymes produced in microorganisms

From: Research, diagnosis and education in inborn errors of metabolism in Colombia: 20 years’ experience from a reference center

Enzyme Disease Expression system Culture condition Enzyme activity (nmol h− 1 mg− 1)
Iduronate-2-sulfate sulfatase IDS (E.C. 3.1.6.13) Hunter (MPS II) Escherichia coli K12 JM 109 100 mL 1.2 to 2.8
Escherichia coli DH5α 100 mL 25.9 to 34.2
Pichia pastoris GS115 100 mL 4.2
1.65 L 29.5
1.65 L (optimized gene) 49.7
N-acetylgalactosamine 6 sulfate sulfatase GALNS (E.C.3.1.6.4) Morquio IVA (MPS IVA) Escherichia coli BL21 (DE3) 3 L 0.067 to 0.078
3 L (improved culture conditions) 6.8
Purified enzyme 2.9
Pichia pastoris GS115 1.65 L 0.02 to 0.09
1.65 L (coexpression of SUMF1) 16.69
β-N-acetylhexosaminidases Hex-A Hex-B, and Hex-S (E.C.3.2.1.52) GM2 Gangliosidoses (Tay Sachs and Sandhoff diseases) Pichia pastoris GS115 1.65 L ( AOX  promoter) Hex-A: 13,124 Hex-B: 12,779 Hex-S: 14,606
Purified enzymes Hex-A: 1.35 × 106 Hex-B: 1.27 × 106 Hex-S: 1.39 × 106
1.65 L (GAP promoter) Hex-A: 32,666
Phenylalanine hydroxylase PAH (EC 1.14.16.1) Phenylketonuria (PKU) Lactobacillus plantarum CM_PUJ411 100 mL Active