Table 4 Summary of recombinant lysosomal enzymes produced in microorganisms
Enzyme | Disease | Expression system | Culture condition | Enzyme activity (nmol h− 1 mg− 1) |
|---|---|---|---|---|
Iduronate-2-sulfate sulfatase IDS (E.C. 3.1.6.13) | Hunter (MPS II) | Escherichia coli K12 JM 109 | 100 mL | 1.2 to 2.8 |
Escherichia coli DH5α | 100 mL | 25.9 to 34.2 | ||
Pichia pastoris GS115 | 100 mL | 4.2 | ||
1.65 L | 29.5 | |||
1.65 L (optimized gene) | 49.7 | |||
N-acetylgalactosamine 6 sulfate sulfatase GALNS (E.C.3.1.6.4) | Morquio IVA (MPS IVA) | Escherichia coli BL21 (DE3) | 3 L | 0.067 to 0.078 |
3 L (improved culture conditions) | 6.8 | |||
Purified enzyme | 2.9 | |||
Pichia pastoris GS115 | 1.65 L | 0.02 to 0.09 | ||
1.65 L (coexpression of SUMF1) | 16.69 | |||
β-N-acetylhexosaminidases Hex-A Hex-B, and Hex-S (E.C.3.2.1.52) | GM2 Gangliosidoses (Tay Sachs and Sandhoff diseases) | Pichia pastoris GS115 | 1.65 L ( AOX promoter) | Hex-A: 13,124 Hex-B: 12,779 Hex-S: 14,606 |
Purified enzymes | Hex-A: 1.35 × 106 Hex-B: 1.27 × 106 Hex-S: 1.39 × 106 | |||
1.65 L (GAP promoter) | Hex-A: 32,666 | |||
Phenylalanine hydroxylase PAH (EC 1.14.16.1) | Phenylketonuria (PKU) | Lactobacillus plantarum CM_PUJ411 | 100 mL | Active |