Fig. 7
From: Molecular diagnosis of anti-laminin 332 (epiligrin) mucous membrane pemphigoid
Use of laminin 332-specific immunoassays in the diagnostic algorithm in mucous membrane pemphigoid. The ELISA with extracellular matrix could represent an addition in patients with a high index of clinical suspicion of mucous membrane pemphigoid showing compatible results by direct immunofluorescence microscopy and either negative or positive findings with dermal binding by indirect immunofluorescence microscopy. A positive result by ELISA with keratinocyte extracellular matrix should be subsequently confirmed by immunoblotting