Fig. 5
From: Molecular diagnosis of anti-laminin 332 (epiligrin) mucous membrane pemphigoid
Reactivity of sera from patients with bullous pemphigoid (BP) by indirect immunofluorescence microscopy. Sera of patients with BP (n = 88) were incubated with sections of human split-skin. Binding of autoantibodies was visualised by using a human IgG4-specific biotinylated secondary monoclonal antibody and AlexaFluo448-labelled streptavidin. The fluorescence intensity was evaluated on a scale from 0 (no binding) to 4 (maximum). Binding of IgG4 autoantibodies from a (a) patient with BP and (b) a healthy donor by indirect immunofluorescence. c Box-plot of epidermal vs. dermal binding of serum autoantibodies in patients with BP