Fig. 4

Comparative reactivity of patients’ sera with native and recombinant laminin 332 by ELISA. a Silver staining of the polyacrylamide gel of the reduced recombinant laminin 332 shows the migration of recombinant laminin 332 at about 260 kDa (β3 chain, 129KDa and γ2 chain, 130KDa). b Receiver-operating-characteristic (ROC) curve. AUC, area under the curve. Test performed with sera from patients with confirmed anti-laminin 332 mucous membrane pemphigoid (n = 36) and controls (n = 116). AUC for native laminin 332 = 0,968 (shown in black). AUC for recombinant laminin 332 = 0,959 (shown in blue). c ELISA reactivity of human sera with the native laminin 332. Scatter plots represent optical density measurements of serum reactivity of confirmed anti-laminin 332 mucous membrane pemphigoid (MMP) (n = 36), normal human sera (n = 116), and possible anti-laminin 332 (n = 26). The cut-off of the assay is represented by a dotted line (cut-off = 0.242, sensitivity = 94.44%, specificity = 84.48%). d ELISA reactivity of human sera with recombinant laminin 332. Scatter plots represent optical density measurements of serum reactivity of confirmed anti-laminin 332 mucous membrane pemphigoid (MMP) (n = 36), normal human sera (n = 116), possible anti-laminin 332 (n = 26), bullous pemphigoid (BP, n = 116), pemphigus vulgaris (PV, n = 116), epidermolysis bullosa acquisita (EBA, n = 116) and dermatits herpetiformis (DH, n = 116). The cut-off of the assay is represented by a dotted line (cut-off = 0.103, sensitivity = 91.67%, specificity = 82.76%)