Fig. 1

(a) Liver tissue section from case 6 Table 1 (Mmalton/Mmalton), with a preserved lobular architecture. Hepatocytes contain eosinophilic inclusions identified as AAT. A few of them mostly located in check-border hepatocytes are centered by dark basophilic material corresponding to calcium precipitates, H.E. 40X, positively stained by von Kossa staining, 100X (Fig. 1a, inset). (b) Liver tissue section from case 1 Table 1 (Mmalton/Mmalton), with fully established cirrhosis, H.E. 40X. The microphotograph shows hepatocytes plenty of cytoplasmic eosinophilic inclusions that were PAS-D positive and immunoreactive with a polyclonal anti-AAT antibody. The largest AAT globule is centered by a dark basophilic material, positively stained by von Kossa staining for calcium