Fig. 5From: Fatal thoracic aortic aneurysm and dissection in a large family with a novel MYLK gene mutation: delineation of the clinical phenotypeKinase activity of the MYLK wild-type (WT) and Ala1491Ser proteins. HeLa cells were transfected with constructs of the WT and mutant MLCK. a. the rate of 32P incorporation into the regulatory light chain (RLC) was measured. The maximal activities of WT (blue circles) and Ala1491Ser (red squares) were obtained at different RLC concentrations. b. Calmodulin (CaM) activation of WT and mutant MLCK proteins. The relative precentage of maximal kinase activity of WT (blue circles) and Ala1491Ser (red squares) was plotted versus various CaM concentrations. The data points represent the mean ± standard error of three or more determinations. The data were fit to the Michaelis-Menten equation for calculation of the Vmax and Km valuesBack to article page