Fig. 5
From: Activation of PKC triggers rescue of NPC1 patient specific iPSC derived glial cells from gliosis
Effect of PMA on p-vimentin and p-GFAP amounts. a Cells were treated with 10 nM PMA and fractions of p-vimentin and b p-GFAP were analysed by semi-quantitative western blot. Treatment with PMA resulted in all NPC1 mutant cell lines in significantly elevated fractions of p-vimentin (N = 4–5, n = 18–30) and p-GFAP (N = 4–5, n = 11–30). Asterisks above bars indicate significance to untreated control and asterisks above lines indicate significances between treated and untreated cells