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Fig. 3 | Orphanet Journal of Rare Diseases

Fig. 3

From: Activation of PKC triggers rescue of NPC1 patient specific iPSC derived glial cells from gliosis

Fig. 3

Induction of gliosis by U18666A. a Unaffected control cells were treated with (b) U18666A to induce cholesterol accumulations shown by Filipin staining (blue). Scale 100 μm. c Calculation of relative fluorescence confirmed higher cholesterol amount in U18666A treated cells (N = 4, n = 6–8). d Increased cholesterol amount in U18666A treated cells was also detected by means of the Amplex red assay (N = 4, n = 7–8). e FACS analysis of GFAP+/vimentin+ cells and f GFAP+/Ki67+ cells revealed an increased amount of reactive astrocytes (N = 4, n = 7–12). g Treatment with U18666A resulted in a reduced amount of p-vimentin and (h) p-GFAP (N = 4, n = 8–12)

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