Fig. 5

Lysosome acidification impairment in chemically-induced Gaucher macrophages. a Representative fluorescence images of THP-1 macrophages cultured for 72 h with CBE + GlcCer (2,5 mM + 200 μM) in the presence of CoQ (25 μM), and stained for 15 min with 10 μg/ml acridine orange. b Quantification of the ratio between the red and green signal of acridine orange was performed by immunofluorescence microscopy using the Image J software. Chemically-induced Gaucher THP-1 macrophages showed reduced red fluorescence and increased green fluorescence and a notably reduction in the red/green signal ratio suggesting decreased lysosomal acidity. Data represent the mean ± SD of three separate experiments. Quantification of the ratio between the red and green signal of acridine orange was performed by immunofluorescence microscopy using the Image J software (n = 100 cells). ^c p < 0.05 between control and chemically-induced Gaucher macrophages. *p < 0.05 between the presence and the absence of CoQ treatment