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Fig. 4 | Orphanet Journal of Rare Diseases

Fig. 4

From: Coenzyme Q10 partially restores pathological alterations in a macrophage model of Gaucher disease

Fig. 4

Increase lysosomal pH is associated with decreased mitochondrial membrane potential (ΔΨm) in chemically-induced Gaucher macrophage. a Representative images of control and chemically-induced Gaucher macrophages stained with LysoSensor Green DND-189 which accumulates in acidic organelles and exhibits green fluorescence, and TMRM, a potentiometric fluorescent indicator that exhibits red fluorescence in mitochondria. Effect of CoQ (25 μM) supplementation for 72 h on lysosomal pH and ΔΨm in chemically-induced Gaucher macrophages. b Determination of lysosomal pH in both control and chemically-induced Gaucher macrophages by staining with LysoSensor Green DND-189 coupled to flow cytometry analysis. Data represent the mean ± SD of three separate experiments. c p < 0.05 between control and chemically-induced Gaucher macrophages. *p < 0.05 between the presence and the absence of CoQ treatment. c Determination ΔΨm was assessed by TMRM staining and flow cytometry analysis. Data represent the mean ± SD of three separate experiments. c p < 0.05 between control and chemically-induced Gaucher macrophages. *p < 0.05 between the presence and the absence of CoQ treatment

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