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Fig. 3 | Orphanet Journal of Rare Diseases

Fig. 3

From: Coenzyme Q10 partially restores pathological alterations in a macrophage model of Gaucher disease

Fig. 3

Impaired autophagic flux in chemically-induced Gaucher macrophages. a Autophagy flux. Determination of LC3-II expression levels in the presence and absence of bafilomycin A1 in control and chemically-induced Gaucher macrophages. Control and chemically-induced Gaucher macrophages were incubated with bafilomycin A1 (100 nM for 12 h). Total cellular extracts were analyzed by immunoblotting with antibodies against LC3. Alpha-tubulin was used as a loading control. b Densitometry of Western blotting was performed using the ImageJ software. Data represent the mean ± SD of three separate experiments. c p < 0.05 between control and chemically-induced Gaucher macrophages. *p < 0.05 between the presence and the absence of CoQ. #p < 0.05 between the presence and the absence of bafilomycin A1

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