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Fig. 2 | Orphanet Journal of Rare Diseases

Fig. 2

From: Coenzyme Q10 partially restores pathological alterations in a macrophage model of Gaucher disease

Fig. 2

Elevation of GlcCer levels and lysosomal markers colocalization in chemically-induced Gaucher macrophages. a Representative images of GlcCer and lysosomal marker LAMP-1 in chemically-induced Gaucher macrophages. THP-1 macrophages were cultured in the presence or absence of CBE + GlcCer (2,5 mM + 200 μM), or CBE + GlcCer + CoQ (2,5 mM + 200 μM + 25 μM) for 72 h. Cells were fixed and immunostained with anti-GlcCer and anti-LAMP-1 (Lysosomal marker) and examined by fluorescence microscopy. b Quantification Image analysis of GlcCer/LAMP-1 puncta in chemically-induced Gaucher macrophages incubated with or without CBE + GlcCer and CBE + GlcCer + CoQ (n = 100 cells). Data represent the mean ± SD of three separate experiments. c p < 0.05 between control and chemically-induced Gaucher macrophages. *p < 0.05 between the presence and the absence of CoQ treatment. c Representative images of Hematoxylin and eosin staining of control, chemically-induced Gaucher macrophages and chemically-induced Gaucher macrophages supplemented with CoQ

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