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Fig. 1 | Orphanet Journal of Rare Diseases

Fig. 1

From: Impaired activity of CCA-adding enzyme TRNT1 impacts OXPHOS complexes and cellular respiration in SIFD patient-derived fibroblasts

Fig. 1

Expression and localization of TRNT1 in patient-derived fibroblasts. a TRNT1 protein expression was analyzed in patient and control fibroblasts by western blot analysis. Tubulin and Tom20 were used as loading controls. b Immunofluorescent imaging of TRNT1 in fibroblast cells. Nuclei are identified by Hoechst stain and mitochondria is stained with an anti-Tom20 antibody (bar = 20 μm). c The expression of TRNT1 in the cytoplasm and nucleus was calculated from images obtained on the Opera automatic confocal microscope. The ratio of the average intensity for TRNT1 signal in the cytoplasm to nucleus was measured in approximately 90 cells per well. The mean ratio per well was averaged over 6 wells and the standard deviation was calculated from the mean ratio values of the 6 wells

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