Fig. 3
From: GNAO1 encephalopathy: further delineation of a severe neurodevelopmental syndrome affecting females
a Homology model of human WT Gαo. The mutated Leu199 is highlighted in yellow space-fill, protein backbone with secondary structure in beige, GDP in red/orange space-fill, magnesium in green. b Structural superposition of mutant Leu199Pro Gαo protein after 1 μ-second of MD simulation (in pink with mutation Leu199Pro shown as red space-fill) and heterotrimeric guanine-nucleotide-binding protein Gi (PDB id: 1GG2, Giα in beige, Giβ in cyan, Giγ in green). c GTPase domain of mutant Leu199Pro Gαo (in pink) compared to WT (in green) after 1 μ-second MD simulations (mutation Leu199Pro shown in red space-fill). d Plot of RMSD (a) for Cα atoms of WT Gαo protein and Leu199pro mutant during 1 μ-second MD simulations (RMSD comparisons made against initial structure)