Fig. 4
From: Bruch’s membrane abnormalities in PRDM5-related brittle cornea syndrome
Changes in extracellular matrix collagens in Bruch’s membrane in PRDM5-associated disease. Immunohistochemistry performed for collagens I, III and IV (red stain, obtained using the XT ultraView Universal Red Alkaline Phosphatase detection system) in retinas of a control individual, and BCS patients P1 and P2 (OM 40×). Absence of staining for collagens I, III and IV in Bruch’s membrane (arrow) is present in sample P1, and absent staining for collagen I with reduced staining for collagens III and IV in P2. Images were recorded and processed identically to allow direct comparisons to be made between them