Fig. 3From: The spectrum of KIAA0196 variants, and characterization of a murine knockout: implications for the mutational mechanism in hereditary spastic paraplegia type SPG8Investigation of potential consequences of heterozygous E430025E21Rik knockout ex vivo. a Quantification of axon length and axonal branching in primary cortical neurons which were stained for the axon marker SMI312 96 h after plating. Scale bars: 20 μm; error bars represent SD. b Visualization of endosomal tubules (arrows) in primary mouse adult fibroblasts by immunofluorescence against the retromer subunit Snx1. The fraction of cells with at least one tubule was determined in 30–40 cells in three independent experiments. Scale bars: 10 μm; error bars represent SDBack to article page