Fig. 2

Histology and immunostaining in the frontal cerebral cortex of CVT, AET and AVT dogs. a–c H&E sections of CVT, AET and AVT showing vacuolation, (d) toluidine blue section of AET showing vacuoles in different cell types, (e–g) ubiquitin immunostaining, (h–j) LFB/PAS staining of deep white matter tracts of frontal cerebral cortex, (k-m) RCA-1 lectin (k-m) and GFAP (n–p) immunostaining staining in frontal cerebral cortex of CVT, AET and AVT. indicate vacuolation, putative microglia with numerous membrane bound vesicles, membrane bound vesicles in neuron, indicate enlarged perivascular space, indicate LFB staining, indicate ubiqutin positive deposits, indicate lectin (RCA-1) staining and indicate GFAP stained astrocytes. Inserts are at 1000× magnification