Fig. 2From: Clinical heterogeneity and potential high pathogenicity of the Mmalton Alpha 1 antitrypsin allele at the homozygous, compound heterozygous and heterozygous statesLiver tissue was obtained by percutaneous liver biopsy of the proband. a Hematein Phloxin Safran (HPS) coloration showing collagen fibrosis around hepatocytes with numerous round eosinophilic cytoplasmic inclusions; (b) PAS staining with diastase digestion showing abundant hyaline globules containing A1AT accumulated within periportal hepatocytes; (c) Immunohistochemical staining using a monoclonal antibody to A1ATBack to article page