Fig. 7

HIF-1α and HIF-2α protein expression is decreased following propranolol treatment. a Western blot assays were performed on lysates from 9XHRE HeLa cells. In agreement with luciferase assays, there were no differences in HIF-1α and HIF-2α protein levels between the different propranolol treatments under normoxia. After DFO treatment (simulating hypoxia), levels of both proteins increased. When propranolol was added to the cells, HIF-1α and HIF-2α levels decreased. b Western blot analysis was performed on lysates from hemangioblastoma cells treated with 50 and 100 μM propranolol for 24 h; HIF-1α and HIF-2α levels were reduced. γ-Tubulin was used as loading control