Patients fibroblasts have been cultured and centrosomes stained as described in Methods. Pictured have been captured using a fluorescence microscope. Examples are presented in Figure 3
A and B. Same cells have been lyzed, and analyzed usinh polyacrylamide gels. Western blats have been performed and probed using an antibody raised against the phosporylated form of CamKII (2C). 1, normal cells ; 2, cells from patient 1 ; 3, cells from patient 3 ; cells from patient 5.