Western blotting and subcellular distribution of endocytic pathway proteins. A. Western blotting: Cells from control (CB) or affected (PA) individual were grown to 80% confluence, and whole cell lysates obtained. Serial dilutions of equal total protein concentrations of the cell extracts were processed to enable quantification, as indicated. B. Subcellular distribution: Cells from control (CB) or affected (PA) individual were grown on glass coverslips, fixed, permeabilized and stained with antibodies to the proteins indicated above each panel. Shown in black and white are structured light epifluorescence image stacks from representative cells, projected into a single plane.