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Figure 3 | Orphanet Journal of Rare Diseases

Figure 3

From: Single point mutation in Rabenosyn-5 in a female with intractable seizures and evidence of defective endocytotic trafficking

Figure 3

Dynamics of transferrin association and dissociation from human fibroblasts. TIRF images of the entire cell from control (A) or affected (B) individual at 300 seconds following addition of fluorescent transferrin. Red rectangles represent region depicted in the time series shown to the right. Transferrin was removed from the medium at t = 600. C. Quantification of the amount of transferrin in the TIRF image of the entire cell, plotted as the mean and SEM of each time point from three individual cells. D. The mean from the data in C was recalculated as a percent of maximal signal. Structured light epifluorescence image stacks from control (E) or affected (F) individual taken at t = 600, projected into a single plane. G. Quantification of signal in the structured light images from 3 individual cells. Statistical significance was calculated by 2-tailed Student t-test. Similar experiments were conducted with three independent passages, with similar results.

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