Figure 6

In vivo effects of different therapeutic approaches were examined in NOD/ scid mice by evaluation of the tumor size each day in the course of the therapy and by calculation of the relation of tumor weight/ mouse weight after tumor dissection at the end of the experiment. Subcutaneous tumors were induced in NOD/scid mice within 18d following injection of about 10⁶ SCCOHT-1^GFP cells. Thereafter, different therapeutic approaches were applied by injection of 200 μl of either 0.9% NaCl, or 5 mM CaCl₂, or 10 μM epothilone B (EpoB), or 5 mM CaCl₂ + 10 μM EpoB each day. A. The relation of tumor weight / mouse weight was calculated after 2d of subsequent injections for control tumors (NaCl) (n = 2) and EpoB-treated tumors (n = 2). B. The relation of tumor weight / mouse weight was calculated after 4d of subsequent injections as the mean ± s.d. for control tumors (NaCl) (n = 5), Ca²⁺-treated tumors (n = 5), and Ca²⁺ + EpoB-treated tumors (n = 4). Statistical analysis was conducted by 1-way ANOVA test (*p = 0.027). C. The tumor size was evaluated each day at 4 consecutive days of subsequent injections as the mean ± s.d. for control tumors (NaCl) (n = 5), Ca²⁺-treated tumors (n = 5), and Ca²⁺ + EpoB-treated tumors (n = 4). Statistical analysis was calculated by unpaired Student's t-test between Ca²⁺-treated tumors and the NaCl-treated tumor sizes as well as between Ca²⁺ + EpoB-treated tumors and the NaCl-treated tumor sizes at the corresponding time points, respectively (*P < 0.05; **P < 0.01).