Figure 4

Comparison of protein expression in SCCOHT-1 and NIH:OVACAR-3 cells. Western blot analysis was performed in SCCOHT-1^GFP and NIH:OVCAR-3^GFP, to compare the protein expression levels of p53, tubulinβ3, and phosphorylated heat shock protein (HSP27) at serine-82 after a 24 h incubation of the 2 cell types with 2 nM of ixabepilone (Ixa), epothilone B (EpoB), methotrexate (MTX), and 5’-fluorouracil (5FU), respectively. The unaltered expression of GAPDH served as a control for equal loading, respectively. Quantification of the blots by densitometry scanning was normalized against the appropriate GAPDH expression and the relative expression levels were documented as bar diagram below the corresponding Western blots.