BMS-204352 effects on Social behaviors. Mice were administered with BMS-204352 2 mg/kg or vehicle (veh) and subjected to social behavioral tests 30 min after the injection. (a) Histograms represent time spent in affiliative behaviors obtained in two laboratories. A three-way ANOVA indicated a non-significant effect of laboratory, but a significant interaction between genotype and treatment. Vehicle-treated Fmr1 KO mice spent less time in affiliative behavior compared to WT, but BMS-204352 injection restored a normal social investigation. (n: WT-veh = 27; WT-2 mg/kg = 16; KO-veh = 25; KO-2 mg/kg = 17). NS, not significant; **p < 0.05 compared to the corresponding WT; ##p < 0.05 compared the corresponding treated group. (b) Illustration of BMS-204352 effect on the three-chamber test by a pseudo-colored heat map representing time spent at each positions related to the social preference trial (fam: familiar mouse, novel: novel mouse). (c) Preference for a conspecific versus an object in the three-chamber test was measured by the time spent in the contact area when a stranger mouse and an object were accessible. Repeat-measures ANOVA indicated that vehicle-treated KO mice like WT shown a preference for the mouse versus the object, and that BMS-204352 treatment had no effect. (d) Preference for a novel versus a familiar mouse, was measured by the time spent in the contact area when a stranger and a familiar mouse were accessible. In vehicle-treated groups a preference for the novel mouse was only observed in WT. This preference was rescued by BMS-204352 treatment in Fmr1 KO mice. (n: WT-veh = 34; WT-2 mg/kg = 19; KO-veh = 42; KO-2 mg/kg = 25). NS, not significant; *p < 0.05, **p < 0.01 and ***p < 0.001 (object versus mouse, familiar versus novel mouse). Data represent mean ± s.e.m.