Figure 5

Overexpression of ARTS lead to stabilization of mutant but not normal GCase. (A) SHSY5Y cells stably expressing WT or N370S mutant GCase were transfected with the indicated amounts of myc-ARTS. After 48 hours cell lysates were prepared and subjected to western blot analysis. The corresponding blot was interacted with anti-myc antibody and with anti-actin antibodies as a loading control. (B) To quantify the results, the blots were scanned and ARTS intensity at each lane was divided by the intensity of actin in the same lane. The value obtained for non-transfected cells was considered as 1. The results represent the mean ± SEM of three independent experiments. **P < 0.005 in Student t-test.