Figure 4

Overexpression of PARIS leads to stabilization of mutant GCase. (A) SHSY5Y cells stably expressing WT or N370S and L444P mutant GCase were transfected with the indicated amounts of GFP-PARIS expressing plasmid. Sixteen hours later, cell lysates were prepared and subjected to western blot analysis. The corresponding blot was interacted with anti-GFP antibody, anti-myc and anti-ERK antibodies, as a loading control. (B) To quantify the results, the blots were scanned and GCase intensity at each lane was divided by the intensity of ERK1/2 in the same lane. The value obtained for WT-GCase was considered as 1. The results represent the mean ± SEM of 3 independent experiments. *P < 0.05, **P < 0.01 in Student t-test.