Effects of medium FCS content on the response to RSV. Cells were treated in 3% or 12% FCS medium with 20 or 75 μM RSV, or vehicle, for 48 h prior to protein extraction and western blot analysis. A) CPT2 protein levels in control or CPT2-deficient fibroblasts. B) VLCAD protein levels in control or VLCAD-deficient fibroblasts. Histograms of protein amounts normalized to ß-actin are shown, as well as representative western-blots. Values are means ± SEM of three westerns. Control values are from three different individuals. *P < 0.05, **P < 0.01, ***P < 0.001 when compared with vehicle-treated cells, $ P < 0.05, $$P < 0.01, $$$ P < 0.001 compared with cells treated in 12% FCS medium.