Figure 2

Time-course of resveratrol uptake and efflux by control and CPT2 (patient 1)- or VLCAD (patient 3)-deficient fibroblasts. A) and B) Cells were incubated at 37°C with tritiated RSV (75 μM) for the indicated times at 37°C. The uptake of labeled RSV was assessed as described in Methods. Data points are means ± SEM of at least three independent experiments each performed in triplicate wells. C) Cells were loaded for 1 h with tritiated RSV (75 μM, 37°C), the labeled medium was removed from the well and replaced by fresh unlabeled medium to measure the remaining cell-associated radioactivity, reflecting RSV efflux at the indicated time points. The values represent means ± SEM relative to uptake value observed after 1 h of treatment (maximal RSV uptake by cells). Data are from a representative experiment among two and each point represents the mean of three determinations on separate wells.