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Table 1 Primary antibodies utilized for immunohistochemistry in the present

From: LC3, an autophagosome marker, is expressed on oligodendrocytes in Nasu-Hakola disease brains

Antibody Supplier Code (ID) Origin Antigen Concentration
LC3 MBL PM036 rabbit recombinant human LC3B spanning amino acid residues 1-120 aa diluted at 1: 5000
BECN1 AnaSpec 54229 rabbit a peptide mapping near the N-terminus of human Beclin-1 0.2 μg/ml
NBR1 ProteinTech 16004-1-AP rabbit recombinant human NBR1-6xHis fusion protein 0.26 μg/ml
HDAC6 Santa Cruz Biotechnology sc-11420 rabbit a peptide spanning amino acid residues 916-1215 of human HDAC6 0.8 μg/ml
p62/SQSTM1 BD Bioscience 610832 mouse a peptide spanning amino acid residues 257-437 of human p62 1 μg/ml
Ubiquitin Dako Z0458 rabbit ubiquitin isolated from bovine erythrocytes 0.25 μg/ml
UBQLN1 Santa Cruz Biotechnology sc-14652 goat a peptide mapping within an internal region of human ubiquilin-1 1 μg/ml
Nogo-A Santa Cruz Biotechnology H-300 rabbit a peptide mapping amino acids 700-1000 of human Nogo-A 0.1 μg/ml
MBP Dako N1564 rabbit MBP purified from human brain prediluted
CNPase Sigma 11-5B mouse purified human CNPase ascites fluid 1:500
Iba1 Wako 019-19741 rabbit a synthetic peptide corresponding to the C-terminus of Iba1 0.5 μg/ml
GFAP Dako N1506 rabbit GFAP purified from bovine spinal cord prediluted
NF Nichirei 412551 (2 F11) mouse NF purified from human brain prediluted
Cleaved CASP3 Cell Signaling Technology #9661 (Asp175) rabbit a peptide mapping amino-terminal residues adjacent to Asp175 of human caspase-3 1:100
  1. Abbreviations: LC3, microtubule-associated protein 1 light chain 3; BECN1, Beclin 1; NBR1, neighbor of BRCA1 gene 1; HDAC6, histone deacetylase 6; SQSTM1, sequestosome 1; UBQLN1, ubiquilin-1; MBP, myelin basic protein; CNPase, 2′,3′-cyclic nucleotide 3′ phosphodiesterase; GFAP, glial fibrillary acidic protein; NF, neurofilament protein; and CASP3, caspase-3.