Table 1 Primary antibodies utilized for immunohistochemistry in the present
From: LC3, an autophagosome marker, is expressed on oligodendrocytes in Nasu-Hakola disease brains
Antibody | Supplier | Code (ID) | Origin | Antigen | Concentration |
|---|---|---|---|---|---|
LC3 | MBL | PM036 | rabbit | recombinant human LC3B spanning amino acid residues 1-120 aa | diluted at 1: 5000 |
BECN1 | AnaSpec | 54229 | rabbit | a peptide mapping near the N-terminus of human Beclin-1 | 0.2 μg/ml |
NBR1 | ProteinTech | 16004-1-AP | rabbit | recombinant human NBR1-6xHis fusion protein | 0.26 μg/ml |
HDAC6 | Santa Cruz Biotechnology | sc-11420 | rabbit | a peptide spanning amino acid residues 916-1215 of human HDAC6 | 0.8 μg/ml |
p62/SQSTM1 | BD Bioscience | 610832 | mouse | a peptide spanning amino acid residues 257-437 of human p62 | 1 μg/ml |
Ubiquitin | Dako | Z0458 | rabbit | ubiquitin isolated from bovine erythrocytes | 0.25 μg/ml |
UBQLN1 | Santa Cruz Biotechnology | sc-14652 | goat | a peptide mapping within an internal region of human ubiquilin-1 | 1 μg/ml |
Nogo-A | Santa Cruz Biotechnology | H-300 | rabbit | a peptide mapping amino acids 700-1000 of human Nogo-A | 0.1 μg/ml |
MBP | Dako | N1564 | rabbit | MBP purified from human brain | prediluted |
CNPase | Sigma | 11-5B | mouse | purified human CNPase | ascites fluid 1:500 |
Iba1 | Wako | 019-19741 | rabbit | a synthetic peptide corresponding to the C-terminus of Iba1 | 0.5 μg/ml |
GFAP | Dako | N1506 | rabbit | GFAP purified from bovine spinal cord | prediluted |
NF | Nichirei | 412551 (2Â F11) | mouse | NF purified from human brain | prediluted |
Cleaved CASP3 | Cell Signaling Technology | #9661 (Asp175) | rabbit | a peptide mapping amino-terminal residues adjacent to Asp175 of human caspase-3 | 1:100 |