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Figure 3 | Orphanet Journal of Rare Diseases

Figure 3

From: Perturbation of specific pro-mineralizing signalling pathways in human and murine pseudoxanthoma elasticum

Figure 3

Immunohistochemical labelling results of the SMAD-RUNX2 pathway in human PXE skin (n = 5 each) and fibroblasts (n = 8 each) compared to controls (n = 3 each) (×10). The top-left panel shows absence of staining in controls (A), positive middermal staining for Alizarin red (B) and RUNX2 (C) in PXE skin. The top-right and middle two panels (D to H) depicts staining for pSmad4 (D), pSmad5 (E), pSmad8 (F) and pSMAD1/5/8 (G) with positive labelling in the middermis of PXE patients compare to no labelling in the negative controls (inserts). The lower panel reveals positive labelling for Alizarin Red (J, K) and ALPL (L, M) in PXE fibroblast cultures (n = 3) compared to controls (n = 3) (I, L). Areas marked with asterisk are enlarged (×40). Scale bar = 100 μm.

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