Figure 12

Immunohistochemical staining results for pERK1/2 (×10). Staining is performed on whisker from the Abcc6 knockout mouse (A-C) and PXE skin tissues (D-F). Alizarin Red stains was used to localize mineralization (A and D). Positive staining for pERK1/2 is obtained on murine whisker (B, arrow) and PXE skin (E, asterisk), co-localizing with mineralization. The pERK1/2 staining being broader than the mineralization staining could represent the active mineralization process where pERK1/2 expression precedes calcification. No staining is noted in control whisker (C) and control skin tissue (F). (n = 5 each). Scale bar = 100 μm.