Figure 4From: Monoclonal antibodies to 65kDa glutamate decarboxylase induce epitope specific effects on motor and cognitive functions in ratsUptake of monoclonal GAD65Ab by the rat mesencephalic cell line AF5. Dose dependent uptake of monoclonal GAD65Ab b78 was assessed by flow cytometry (A). AF5 cells were incubated for 4 hours with 10 (blue line) and 50 μg (green line) Alexa-488 b78. Unstained cells are shown in red. Cellular localization of GAD65Ab b78 and b96.11, and monoclonal antibody HAA1-Alexa 647 was assessed by fluorescence and Bright Field microscopy (B). AF5 cells were incubated for 4 hours with Alexa-488 b78, Alexa-488 b96.11 or HAA1-Alexa 647 followed by incubation with SYTOX-orange for identification of dead cells. The images reported in the figures are representative of at least three experiments that gave similar results.Back to article page