Uptake of monoclonal GAD65Ab by the rat mesencephalic cell line AF5. Dose dependent uptake of monoclonal GAD65Ab b78 was assessed by flow cytometry (A). AF5 cells were incubated for 4 hours with 10 (blue line) and 50 μg (green line) Alexa-488 b78. Unstained cells are shown in red. Cellular localization of GAD65Ab b78 and b96.11, and monoclonal antibody HAA1-Alexa 647 was assessed by fluorescence and Bright Field microscopy (B). AF5 cells were incubated for 4 hours with Alexa-488 b78, Alexa-488 b96.11 or HAA1-Alexa 647 followed by incubation with SYTOX-orange for identification of dead cells. The images reported in the figures are representative of at least three experiments that gave similar results.