Figure 4

14q32 orthologous region transcript levels and outcome in canine osteosarcoma. (A) miR-134 and B) miR-544 expression levels determined by qRT-PCR in canine osteosarcoma primary tumor samples with clinical follow-up information. The miRNA expression levels were normalized relative to reactive canine osteoblasts. Osteosarcoma samples were ranked based on expression levels of miR-134 from highest to lowest. Samples were grouped based on whether they were above or below the median expression levels. (C and D) Kaplan-Meier analysis of survival in osteosarcoma patients based on miR-134 and miR-544 expression. Survival of dogs with osteosarcoma in Group 1 and Group 2 are significantly different (miR-134 p-value 0.004, miR-544 p-value 0.01). Dogs with lowest levels of miR-134 or miR-544 expression showed decreased likelihood of survival.