Figure 2

Prognostic significance of genes correlated with 14q32 miRNAs in canine osteosarcoma. (A) Unsupervised clustering of human mRNAs (n = 385) that shows high-level direct correlations to miR-382 for two normal bone tissues and 11 osteosarcoma tumors, for which we have both mRNA profiles and miRNA profiles. A positive correlation to miR-382 is shown in yellow (n = 288), and a negative correlation to miR-382 is shown in blue (n = 97), at the right of the heatmap. Directional Functional enrichment analyses (Ingenuity Pathways Analyses) shows that correlating transcripts are enriched in transcripts involved in metastasis, and direction of change is consistent with increased activity of metastatic function in tumors with decreased levels of 14q32 miRNA member, miR-382. Identities of positive and negatively correlating mRNA are provided in Additional file 6: Table S5 and Additional file 7: Table S6. (B) Unsupervised hierarchical clustering heatmap of canine mRNA found in 26 canine osteosarcoma-derived samples that correspond to human mRNAs correlating to miR-382. The yellow and blue bars represent correlations to miR-382 (positive = yellow or negative = blue) observed in the human data indicating that the direction of change shows similar trends between human and canine data.(C) Kaplan-Meier survival curves generated using the groups shown in 2B. Survival times are significantly different between the two groups of tumors (p-value 0.02).