Figure 2
From: miR-411 is up-regulated in FSHD myoblasts and suppresses myogenic factors
Cellular localization of miR-411 in FSHD myoblasts. The cellular localization of miR-411 was determined by in situ hybridization using LNA probes. While not seen in control myoblasts (A), the expression of miR-411 was visible in the cytoplasm of some FSHD myoblasts (B: marked by asterisks). The negative control using probes with scramble sequences did not generate any signal in control myoblasts (C) or FSHD myoblasts (D). In contrast, the positive control (U6 probe) showed nuclear staining in both control myoblasts (E) and FSHD myoblasts (F). The scale bar is 50 μm.