Transient expression of wildtype and mutant ARSB cDNA constructs. COS-7 cells were transfected with wildtype (wt) and mutant cDNA constructs. ‘mock’ indicates that the cells were transfected with a cDNA construct containing beta-hexosaminidase cDNA. SDS-PAGE followed by immunoblotting was performed and polyclonal rabbit anti-galsulfase serum was used to visualize ARSB. Equal amounts of protein were loaded per lane. The figure at the top shows the result of a representative experiment obtained at 72 hours after transfection. The figure at the bottom shows the ARSB activity as percentage of wild type ARSB activity as measured in cell homogenates at 48 hours after transfection.