Figure 7

Evaluation of cell death after exposure to 2-MCA, MMA and PA. A; left panel: Immunohistochemical staining for cleaved caspase-3 (red signal). Scale bar: 100 μm. A; right panel: Representative western blots with data quantification of whole-cell lysates for full length caspase-3 and the large fragment of cleaved caspase-3 (i.e. activated caspase-3) on DIV 8 (left) and on DIV 14 (right). Actin was used as a loading control. The quantifications of cleaved caspase-3 normalized to actin are expressed as percentage of respective controls. The values represent the mean ± SEM from three replicates taken from two independent experiments. B: LDH in culture medium of cultures from DIV 8 (left) and DIV 14 (right). Mean ± SEM of seven replicate cultures assessed by Student’s t-test; *** p<0.001.