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Figure 4 | Orphanet Journal of Rare Diseases

Figure 4

From: Brain damage in methylmalonic aciduria: 2-methylcitrate induces cerebral ammonium accumulation and apoptosis in 3D organotypic brain cell cultures

Figure 4

Effects of 2-MCA, MMA and PA on oligodendrocytes. Immunohistochemical staining for galactocerebroside (GalC, DIV 8) and myelin basic protein (MBP, DIV 14) on cryosections of cultures on DIV 8 and 14. Cultures were exposed to 1 mM 2-MCA, MMA or PA (A; left panel) or lower concentrations of 2-MCA (B). Scale bar: 100 μm. A; right panel: Representative western blots with data quantification of whole-cell lysates for MBP on DIV 14. Actin was used as a loading control. The quantifications of MBP levels normalized to actin are expressed as percentage of respective controls. The values represent the mean ± SEM from three replicates taken from two independent experiments.

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Orphanet Journal of Rare Diseases

ISSN: 1750-1172