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Figure 3 | Orphanet Journal of Rare Diseases

Figure 3

From: Brain damage in methylmalonic aciduria: 2-methylcitrate induces cerebral ammonium accumulation and apoptosis in 3D organotypic brain cell cultures

Figure 3

Effects of 2-MCA, MMA and PA on astrocytes. Immunohistochemical staining for glial fibrillary acidic protein (GFAP) on cryosections of cultures on DIV 8 and 14. Cultures were exposed to 1 mM 2-MCA, MMA or PA (A; left panel) or lower concentrations of 2-MCA (B). Swollen proximal fibers are indicated by black arrows. Scale bar: 100 μm. A; right panel: Representative western blots with data quantification of whole-cell lysates for GFAP on DIV 8 (above) and on DIV 14 (below). Actin was used as a loading control. The quantifications of GFAP levels normalized to actin are expressed as percentage of respective controls. The values represent the mean ± SEM from three replicates taken from two independent experiments.

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