Skip to main content
Figure 1 | Orphanet Journal of Rare Diseases

Figure 1

From: Brain damage in methylmalonic aciduria: 2-methylcitrate induces cerebral ammonium accumulation and apoptosis in 3D organotypic brain cell cultures

Figure 1

Treatment protocols. Brain cell aggregates were exposed to 1 mM 2-MCA, MMA and PA or lower concentrations of 2-MCA at two time points representing different developmental stages of brain cell maturation (Protocols A and B). Metabolites were added 6 times every 12 hours (indicated by arrows) starting on DIV 5 in protocol A and on DIV 11 in protocol B (treatment DIVs are indicated by black boxes) 12 hours after the change of the medium. Aggregates were harvested 5 hours after the last treatment on DIV 8 in protocol A and on DIV 14 in protocol B.

Back to article page

Orphanet Journal of Rare Diseases

ISSN: 1750-1172