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Figure 6 | Orphanet Journal of Rare Diseases

Figure 6

From: Quantitative proteomic analysis of cultured skin fibroblast cells derived from patients with triglyceride deposit cardiomyovasculopathy

Figure 6

SRM and qPCR analysis of two selected differentially expressed proteins. (A) Comparison of PLIN2 expression in SILAC proteomics with that of microarray transcriptomics using the same format as described in the legend of Figure 5. PLIN2 expression is elevated at protein levels but not transcript levels. (B) SRM analysis confirmed over-expression of PLIN2 at protein levels. (C) qPCR analysis confirmed that expressions of PLIN2 were not so different at transcript levels. Characters at the bottom indicate cell names. (D) Comparison of FLG expression in SILAC analysis with that of microarray transcriptomics using the same format as described in the legend of Figure 5. (E) SRM analysis confirmed over-expression of FLG at protein levels. (F) Quantitative PCR analysis confirmed over-expression of FLG at transcript levels. Characters at the bottom indicate cell names.

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Orphanet Journal of Rare Diseases

ISSN: 1750-1172