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Figure 6 | Orphanet Journal of Rare Diseases

Figure 6

From: Induced pluripotent stem cells from patients with human fibrodysplasia ossificans progressiva show increased mineralization and cartilage formation

Figure 6

Increased mineralization capacity in integration-free FOP iPS cells. (A) eFOP iPS cells show greater mineralization than controls by von Kossa staining. (B) Quantitation of mineralization. n = 46–51 wells for each condition, combining at least three replicates of each of the four control iPS lines or five FOP iPS lines. Error bars, +/− 1 SD. *, p < 0.05. †, p < 0.1. (C) Quantitative PCR of ACVR1 (wildtype and R206H mutant) during osteogenic culture. (D–F) Quantitative PCR during osteogenic culture of genes downstream of BMP signaling (ID1 and DLX5), chondrogenic (SOX9, ACAN, COL2a1, and COMP), osteogenic (RUNX2, SP7, and OC), and mineralization (AP and TFIP11) markers. n = 3–6 samples per iPS cell line per condition, each measured in triplicate; Error bars, +/− 1 SEM; *, p < 0.05. †, p < 0.1.

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