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Figure 1 | Orphanet Journal of Rare Diseases

Figure 1

From: Induced pluripotent stem cells from patients with human fibrodysplasia ossificans progressiva show increased mineralization and cartilage formation

Figure 1

Characterization of retroviral iPS cell lines. (A) Representative photos of FOP fibroblasts. (B) Subsequent retroviral iPS cell colonies on mouse feeder cells (colony indicated by dashed circle). (C) Retroviral iPS cell colonies after transition to feeder-free conditions. Black scale bar = 50 μm. (D) Retroviral iPS cell lines are heterozygous for the ACVR1 R206H (617 G > A) mutation. Control lines vWTa and vWTb1 showed a wildtype ACVR1 gene. (E) The OCT3/4 and C-MYC retroviral transgenes are silenced in the human iPS cells, but KLF4 and SOX2 show detectable levels of expression in some lines. (F) Pluripotent iPS cells show higher expression of pluripotency markers TERT and NANOG as compared to differentiated cultures (15-day-old EBs). Gene expression is normalized to GAPDH and relative to H9 differentiated EB levels. Error bars are average expression +/− 1 SD of technical triplicates. Gene expression studies were repeated in triplicate. (G) Teratoma images showing representative tissues from all three germ layers. Yellow scale bar = 200 μm.

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