Table 1 Cell types and molecules evaluated by flow cytometry in this study
From: GPI-anchor and GPI-anchored protein expression in PMM2-CDG patients
Cell type | Molecule | GPI-anchor | MoAb | Source |
|---|---|---|---|---|
Erythrocytes | CD55 | Yes | 1A10 (PE) | Becton-Dickinson |
CD59 | Yes | MEM-43 (PE) | Caltag | |
Monocytes | CD14 | Yes | MФP9 (PE) | Becton-Dickinson |
61D3(FITC) | eBioscience | |||
CD55 | Yes | 1A10 (PE) | Becton-Dickinson | |
CD48 | Yes | J4-57 | Beckman-Coulter | |
CD33 | No | WM53 (PE) | BD Pharmingen | |
FLAER | GPI | --(Alexa 488) | Pinewood Scientific | |
Lymphocytes | CD48 | Yes | J4-57 | Beckman-Coulter |
CD3 | No | SK7 (FITC) | Becton-Dickinson | |
CD19 | No | HIB19(PE-Cy5) | BD Pharmingen | |
CD16 | No | 3G8 (PE) | BD Pharmingen | |
FLAER | GPI | --(Alexa 488) | Pinewood Scientific | |
Neutrophils | CD16 | Yes | KD1 | Culture supernatant |
3G8 (PE) | BD Pharmingen | |||
3G8 (PE-Cy5) | BD Pharmingen | |||
CD55 | Yes | 1A10 (PE) | Becton-Dickinson | |
CD24 | Yes | ML5 (PE) | BD Pharmingen | |
CD66 | Yes | Kat4c | Dako | |
CD54 | No | HA58 (PE) | BD Pharmingen | |
FLAER | GPI | --(Alexa 488) | Pinewood Scientific |