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Table 1 Cell types and molecules evaluated by flow cytometry in this study

From: GPI-anchor and GPI-anchored protein expression in PMM2-CDG patients

Cell type Molecule GPI-anchor MoAb Source
Erythrocytes CD55 Yes 1A10 (PE) Becton-Dickinson
CD59 Yes MEM-43 (PE) Caltag
Monocytes CD14 Yes MФP9 (PE) Becton-Dickinson
61D3(FITC) eBioscience
CD55 Yes 1A10 (PE) Becton-Dickinson
CD48 Yes J4-57 Beckman-Coulter
CD33 No WM53 (PE) BD Pharmingen
FLAER GPI --(Alexa 488) Pinewood Scientific
Lymphocytes CD48 Yes J4-57 Beckman-Coulter
CD3 No SK7 (FITC) Becton-Dickinson
CD19 No HIB19(PE-Cy5) BD Pharmingen
CD16 No 3G8 (PE) BD Pharmingen
FLAER GPI --(Alexa 488) Pinewood Scientific
Neutrophils CD16 Yes KD1 Culture supernatant
3G8 (PE) BD Pharmingen
3G8 (PE-Cy5) BD Pharmingen
CD55 Yes 1A10 (PE) Becton-Dickinson
CD24 Yes ML5 (PE) BD Pharmingen
CD66 Yes Kat4c Dako
  CD54 No HA58 (PE) BD Pharmingen
  FLAER GPI --(Alexa 488) Pinewood Scientific
  1. MoAb: Monoclonal antibody.