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Figure 1 | Orphanet Journal of Rare Diseases

Figure 1

From: Passive transfer of collagen XVII-specific antibodies induces sustained blistering disease in adult mice

Figure 1

Recombinant fragments of murine BP180/ CXVII. (a) BP180/ CXVII is composed of an intracellular N-terminal fragment localized to the hemidesmosomal plaque of basal keratinocytes with a long extracellular portion containing 15 collagenous domains (filled rectangles) separated by non-collagenous regions protruding into the basal membrane. Four fragments of murine type XVII collagen cDNA cloned in pGEX-6P-1 were expressed in E. coli. Amino acid positions are shown below each fragment (the diagram is not at scale). (b) When run on an SDS-PAGE gel the proteins migrated correspondingly to their calculated molecular weights of 37, 32, 39 and 57 kDa, respectively. In lanes 1 through 6 we loaded: molecular weight marker, GST, GST-mCXVII-EC1, GST-mCXVII-EC3, GST-mCXVII-EC7 and GST-mCXVII-IC2. The arrow indicates GST-mCXVII-IC2 at 57kDa and the arrowhead indicates GST at 27kDa.

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