Quantitative mass spectrometric analysis of HexCer (sum of GlcCer and GalCer). (A) Total HexCer amount in liver, spleen, and brain of 6 -month-old GBA2-deficient (KO) and wild-type (WT) mice. (B) Amount of the HexCer species carrying the typical neuronal ceramide anchor with a stearic acyl residue. *: P < 0.05, n = 4 animals per group. (C) TLC of a representative brain lipid sample in which GlcCer and GalCer are separated. Note the increased GlcCer in the KO. The double band reflects heterogeneity of its ceramide anchor composition.