Table 1 PCR primer sequences, amplicon length and PCR reaction temperature
From: A pilot newborn screening program for Mucopolysaccharidosis type I in Taiwan
Exon | Primer sequence | Amplicon length (bp) | Annealing temperature (°C) |
|---|---|---|---|
1 | F:5′-CCGCAGTCCCGAGCAC-3′ | 277 | 60 |
R:5′-GCTCCGGTCTCTGAAGCTCT-3′ | |||
2 | F: 5′-CCCTCGTCTTACTGCTGCTG-3′ | 401 | 60 |
R: 5′TCCCATCTGTGCCTCTGTAA-3′ | |||
3,4 | F: 5′-CATACCAGGCCTTCATAGGG-3′ | 708 | 58 |
R: 5′CCAACCTATCCCTTGTCACC-3′ | |||
5 | F: 5′-CATCACCTTGCACCCTCC-3′ | 273 | 60 |
R: 5′-CGTCTACACCTGCCCTGG-3′ | |||
6 | F: 5′-CCGCTCATCCCCAGGGCAGGTGTA-3′ | 301 | 60 |
R: 5′-ACAGCGGCTGAGGGCGCAGAACAC-3′ | |||
7 | F: 5′-CATCTCCCTCCACAGGAAGGTG-3′ | 500 | 60 |
R: 5′-GGTAGCTCAGGAAGGCATTGTC-3′ | |||
8 | F: 5′-TTCCTCCCGAGACGGGACAGGCGA-3′ | 437 | 60 |
R: 5′-CTCCCCTTGGTGAAGGAGTC-3′ | |||
9 | F: 5′-TGGGGACTCCTTCACCAAGGGGAG-3′ | 370 | 60 |
R: 5′-CAGAGCCCCAGCGGGGCCAGAGAC-3′ | |||
10 | F: 5′-ATCTACGCGAGCGACGAC-3′ | 470 | 60 |
R: 5′-GGTCCTCAGGGTTCTCCAG-3′ | |||
11 | F: 5′-GTGTGGGTGGGAGGTGGAGCGGTG-3′ | 302 | 60 |
R: 5′-AGGGAAGGGCTGTGAGGCGTCGG-3′ | |||
12 | F: 5′-GCTTTTGCTGGTGCACGTGT-3′ | 295 | 60 |
R: 5′-AAGTGGCCCGAGTGACCGCAT-3′ | |||
13,14 | F: 5′-CCTAGGGGACATGAGATGGA-3′ | 814 | 58 |
R: 5′-CGGGGTTTACCCTTGGAG-3′ |