ACTH-independent macronodular adrenocortical hyperplasia reveals prevalent aberrant in vivo and in vitroresponses to hormonal stimuli and coupling of arginine-vasopressin type 1a receptor to 11β-hydroxylase

Table 4 Forty-eight hour in vitro cortisol responses to hormonal stimuli in AIMAH cells

	Dose	n	Partial response n (%)	Full response n (%)	Correlation with *in vivo*test	Correlation with t=2 h
ACTH_1-24	10 ng/ml	7	0	6 (86%)	−0.39	0.20
AngII	100 nM	4	0	4 (100%)	1.00**^#	n.a.
AVP	10 nM	3	0	2 (67%)	0.50^†; n.a.^#	n.a.
AVP	100 nM	7	1 (14%)	6 (86%)	−0.18^†; -0.086^#	−0.50
Desmopressin	10 nM	5	0	0	−0.67	1.00**
Metoclopramide	1 μM	4	0	2 (50%)	0.60	1.00**
Norepinephrine	1 μM	4	0	0	−0.50^#	−0.50^#
hCG	100 IU/ml	4	0	0	−0.60	−0.50
TSH	10 nM	2	0	1 (50%)	n.a.	n.a.
Glucagon	1 μM	4	0	0	−0.20	n.a.
GIP	100 nM	2	0	0	−1.00^¶	n.a.

**p<0.01, ^¶ standard mixed meal, ^# upright posture test, ^† AVP im, n.a. not applicable.

ISSN: 1750-1172